SHEARS

Sequence-guided editing analysis and ranking

This version focuses on structured input, sequence validation, ranked ORF analysis, and a guided workflow that can support later design modules.

Organism: HumanOutcome: KnockoutModality: LoadingNuclease: spcas9Input: Manual sequenceWorkspace: 0 loaded

Current Focus

No active manual sequence

Loading workflow options.

Workspace Sequences

No sequences are loaded in the workspace.

Non-active sequences will appear here after you load them into the workspace.

Sequence Intake

Configure the current run

Choose the workflow, load or analyze the active sequence, and score guides when the target looks right.

Loading workflow options.

Step 1: Select organism

Applies on the next analysis run.

Step 2: Select workflow and nuclease

Step 3: Provide sequence input

Separate multiple sequences with one blank line if you want to load them into the workspace as separate items.

Upload a FASTA file, paste one or more sequences, or load a sequence into the workspace.

Active Input

Review the active sequence

Confirm the current target and context before you spend time on guide scoring.

Analyze a manual sequence to establish the active sequence for this run.

Ranked ORFs

Choose the guide-analysis target

Keep the full sequence when you want broad coverage, or switch to a focused coding region when that is the better scoring target.

Analyze an input first to review ORFs and choose the guide-analysis target region.

Guide Review

Score, compare, and stage guides

Score guides for the active target, select the ones you want to keep, and export the current session when ready.

Fast mode refines the initial top 16 guides without rescue and keeps the shortlist fully refined.

Analyze the active input, then score passing guides.

Scored candidates and guide-detail surfaces will appear here after the active input is scored.